Journal: Journal of Physiology and Biochemistry

Article Title: Modulation of SIRT1/PPARγ pathways and tight junction proteins by nicotinamide riboside under chronic variable stress

doi: 10.1007/s13105-026-01153-7

Figure Lengend Snippet: The effects of different nicotinamide riboside doses on the levels of PepT1 ( A ), LAT2 ( B ), EAAT3 ( C ), FABP2 ( D ), and FATP4 ( E ) proteins in the jejunum tissue of rats under normal and chronic variable stress (CVS) conditions. Western blot analysis was performed using β-actin as a loading control to ensure equal protein loading. All bands are shown in (G). Different letters (a–e) indicate statistically significant differences between groups ( p < 0.05) according to Tukey’s post hoc test following two-way ANOVA. Two-way ANOVA revealed significant effects for PepT1 (Condition p < 0.0001; NR p = 0.0001; Condition x NR p < 0.0001), LAT2 (Condition p < 0.0001; NR p = 0.0002; Condition x NR p = 0.0007), EAAT3 (Condition p < 0.0001; NR p < 0.0001; Condition x NR p < 0.0001), FABP2 (Condition p < 0.0001; NR p < 0.0001; Condition x NR p = 0.0236), and FATP4 (Condition p < 0.0001; NR p < 0.0001; Condition x NR p = 0.3521). Comprehensive statistical data are presented in Supplementary Table and full immunoblots images are shown in supplementary Fig.

Article Snippet: Membranes were blocked with 5% bovine serum albumin (BSA) for 2 h at room temperature and incubated overnight at 4 °C with primary antibodies as follows: PPARγ (sc-271392), GLUT2 (sc-518022), IRS-1 (sc-8038), SIRT1 (sc-74465), FASN (sc-48357), claudin-1 (sc-166338), claudin-4 (sc-376643), occludin (sc-133256), ZO-1 (sc-33725), MUC2 (sc-53381), PepT1 (sc-373742), LAT2 (sc-293242), FABP2 (sc-374482), FATP4 (sc-393309) and β-Actin (sc-517582) (all from Santa Cruz Biotechnology); SGLT1 (DF7202-100; Affinity Biosciences); EAAT3 (orb1161904; Biorbyt Ltd., Cambridge, UK).

Techniques: Western Blot, Control

Journal: Animal Nutrition

Article Title: New functions of enzymatic cottonseed protein: Improvement of intestinal digestion and absorption, structure and microbial composition of juvenile yellow catfish ( Pelteobagrus fulvidraco )

doi: 10.1016/j.aninu.2025.03.022

Figure Lengend Snippet: Effects of different levels of dietary enzymatic cottonseed protein (ECP) on the mRNA expression level of transporters (A) and the protein expression level of PepT1 (B). NP = normal protein (42% CP) diet; ECP0 = supplement 0% ECP to low protein (LP) (39% CP) diet; ECP1 = supplement 1% ECP to LP diet; ECP2 = supplement 2% ECP to LP diet; ECP3 = supplement 3% ECP to LP diet; ECP4 = supplement 4% ECP to LP diet; ECP5 = supplement 5% ECP to LP diet. One-way ANOVA was used for data from the ECP0-5 groups, and data from the NP group were compared with those from the ECP0 and ECP2 groups using Independent Samples T -tests. Mean values from the ECP0-5 groups with different letters were significantly different ( P < 0.05); ∗ indicated a significant difference between the NP group and ECP0 group ( P < 0.05) Values are means ( n = 6).

Article Snippet: It was then incubated for 17 h at 4 °C with primary antibodies including PepT1 (ER64550), caudal type homeobox transcription factor 2 (CDX2) (ET1605-4), PDZ domain containing 1 (PDZK1) (HA722729), and Rho-associated kinase 1 (ROCK1) (ER40122) from Huabio Biotechnology Co., Ltd., Hangzhou, Zhejiang, China, and specificity protein 1 (SP1) (A14662) and sodium/hydrogen exchange isoform 3 (NHE3) (A17591) from ABclonal Biotechnology Co., Ltd., Wuhan, Hubei, China.

Techniques: Expressing

Journal: Animal Nutrition

Article Title: New functions of enzymatic cottonseed protein: Improvement of intestinal digestion and absorption, structure and microbial composition of juvenile yellow catfish ( Pelteobagrus fulvidraco )

doi: 10.1016/j.aninu.2025.03.022

Figure Lengend Snippet: Effects of different levels of dietary enzymatic cottonseed protein (ECP) on the genes nrf2 (A) and jak2 (B), and proteins SP1 (C), CDX2 (D), NHE3 (E), and PDZK1 (F) expression of PepT1-related. NP = normal protein (42% CP) diet; ECP0 = supplement 0% ECP to low protein (LP) (39% CP) diet; ECP1 = supplement 1% ECP to LP diet; ECP2 = supplement 2% ECP to LP diet; ECP3 = supplement 3% ECP to LP diet; ECP4 = supplement 4% ECP to LP diet; ECP5 = supplement 5% ECP to LP diet. One-way ANOVA was used for data from the ECP0-5 groups, and data from the NP group were compared with those from the ECP0 and ECP2 groups using Independent Samples T -tests. Mean values from the ECP0-5 groups with different letters were significantly different ( P < 0.05); ∗ indicated a significant difference between the NP group and ECP0 group ( P < 0.05); # indicated a significant difference between the ECP0 group and ECP2 group ( P < 0.05). Values are means ( n = 6).

Article Snippet: It was then incubated for 17 h at 4 °C with primary antibodies including PepT1 (ER64550), caudal type homeobox transcription factor 2 (CDX2) (ET1605-4), PDZ domain containing 1 (PDZK1) (HA722729), and Rho-associated kinase 1 (ROCK1) (ER40122) from Huabio Biotechnology Co., Ltd., Hangzhou, Zhejiang, China, and specificity protein 1 (SP1) (A14662) and sodium/hydrogen exchange isoform 3 (NHE3) (A17591) from ABclonal Biotechnology Co., Ltd., Wuhan, Hubei, China.

Techniques: Expressing